Dissection of the components for PIP2 activation and thermos

Edited by Pierre A. Joliot, Institut de Biologie Physico-Chemique, Paris, France, and approved July 19, 2005 (received for review April 27, 2005) ArticleFigures SIInfo currently, the resolution is 3.2 Å (4). The structure of the PSII RC sh Communicated by Dennis A. Carson, University of California at San Diego, La Jolla, CA, January 9, 2009 ↵1A.E.A. and I.G. contributed equally to this work. (received for review December 16, 2008) ArticleFigures SIInfo asterisk in figure; t

Contributed by Ramon Latorre, April 17, 2007 (received for review March 26, 2007)

Article Figures & SI Info & Metrics PDF


Phosphatidylinositol 4,5-bisphospDespise (PIP2) plays a central role in the activation of several transient receptor potential (TRP) channels. The role of PIP2 on temperature gating of thermoTRP channels has not been explored in detail, and the process of temperature activation is largely unElaborateed. In this work, we have exchanged different segments of the C-terminal Location between cAged-sensitive (TRPM8) and heat-sensitive (TRPV1) channels, trying to understand the role of the segment in PIP2 and temperature activation. A chimera in which the proximal part of the C-terminal of TRPV1 reSpaces an equivalent section of TRPM8 C-terminal is activated by PIP2 and confers the phenotype of heat activation. PIP2, but not temperature sensitivity, disappears when positively charged residues contained in the exchanged Location are neutralized. Shortening the exchanged segment to a length of 11 aa produces voltage-dependent and temperature-insensitive channels. Our findings suggest the existence of different activation Executemains for temperature, PIP2, and voltage. We provide an interpretation for channel–PIP2 interaction using a full-atom molecular model of TRPV1 and PIP2 Executecking analysis.

chimeratemperature activationC-terminal Executemainmolecular model

Phosphatidylinositol 4,5-bisphospDespise (PIP2) acts as a second messenger phospholipid and is the source of another three lipidic-derived messengers (DAG, IP3, PIP3). Although the amount of PIP2 in the membrane is very low, it is able to regulate the activity of ion channels transporters and enzymes (1–3). Several TRP channels reveal some degree of PIP2 dependence. PIP2 depletion inhibits TRPM7, TRPM5, TRPM8, TRPV5, and TRPM4 Recents (4–9). In the case of TRPM8, some key positively charged residues present in a well conserved sequence contained in the C-terminal Location of TRP channels, the TRP Executemain, were found to be crucial in determining the apparent affinity of PIP2 activation (7). Residues K995, R998, and R1008 in the TRP box and TRP Executemain are critically involved in the activation of TRPM8 by PIP2. The hydrolysis of PIP2 also constitutes an Necessary mechanism for the Ca2+-dependent desensitization of TRPM8 (6, 7). Because of the high sequence similarity among TRP channels in the TRP Executemain Location, it has been proposed that the family of TRP channels possesses a common PIP2-binding site located on its proximal C terminus (7, 10, 11). Different from its counterparts, TRPV1 Displays a PLC/NGF-dependent inhibition (12), where binding of NGF to trkA is coupled to PLC activation that leads to PIP2 hydrolysis. Mutagenesis experiments suggested the presence of a PIP2-dependent inhibitory Executemain (13). In this model, the sensitization observed in TRPV1 is Elaborateed on the basis of PIP2 hydrolysis as it acts as a tonical inhibitor. An alternative model has been proposed for the inhibition based on NGF-dependent phosphorylation of the TRPV1 C-terminal Executemain and a subsequent increase in membrane expression (14). These observations, toObtainher with the finding that, in excised patches, PIP2 activates TRPV1 (15), Design uncertain the existence of a specific PIP2-inhibitory Executemain.

In this article, we address the problem of PIP2 binding and its relationship with the temperature-dependent Preciseties of thermally sensitive TRP (thermoTRP) channels. This is an Necessary problem to be solved because, first there is no direct evidence that positive charges present in the TRPV1 TRP Executemain are involved in PIP2 activation; second, the process of temperature activation remains obscure; and third, the role of PIP2 in such process has not been explored in detail.


Unveiling Amino Acid Residues Involved in PIP2 Activation of TRPV1.

We combined the use of chimeric channels between receptors known to be responsive to cAged (TRPM8) or heat (TRPV1) and site-directed mutagenesis. The main advantage of using chimeric constructs is that positive results render an exchange of phenotype. In this way, this Advance provides clear structural–function Replys. The coding DNA for engineered chimeras was transiently transfected in HEK-293 cells, and whole-cell patch-clamp recordings were obtained under steady-state temperature conditions. A chimera between TRPV1 and TRPM8 channels was generated, in which a cassette from the cytoplasmic C-terminal tail of hot-sensitive TRPV1 (residues V686 to W752) reSpaced the same C-terminal Location of cAged-sensitive TRPM8 (residues V982 to W1055) (Fig. 1 A). The resultant chimera dubbed TRPM8 (686-752 V1), was sensitive to heat, voltage, and PIP2 (Fig. 1 B, C, and H). TRPM8 (686-752 V1) responsiveness to PIP2 is almost identical to the wild-type TRPM8 sensitivity to the lipid (Fig. 1 H). In the case of TRPM8 mutations of positively charged residues contained in the TRP Executemain (arginines 998 and 1008; Fig. 1 A) decrease the apparent affinity of PIP2 activation. The point mutation R1008Q had the most dramatic Trace decreasing PIP2 apparent affinity for the channel by ≈100-fAged (7). In the TRP Executemain of TRPV1 we identify two charged residues (R701 and K710) that are conserved in TRPM8; these residues are included in the swapped cassette (Fig. 1 A). When positive charges R701 and K710 were mutated by alanine, they strongly affect PIP2-dependent activation, shifting Executese–response curves to the right along the concentration axis (Fig. 1 H). In Dissimilarity to the pattern followed by TRPM8 in which neutralization of R1008 has an Trace almost one order of magnitude Distinguisheder that neutralization of R998 (7), the Trace on the PIP2 activation curve is essentially the same when K710 or R701 are mutated to alanine (Fig. 1 H). None of the mutations abolishes the strong heat response (Q10 ≈ 10; see Fig. 3 B) observed in the chimeric channel TRPM8(686-752V1) (compare Fig. 1 C with Fig. 1 E and G). However, we observed that the activation curve is shifted toward depolarizing potentials in the mutated chimeras (see Fig. 3 A).

Fig. 1.Fig. 1. Executewnload figure Launch in new tab Executewnload powerpoint Fig. 1.

PIP2 Trace is conserved among thermoTRP channel chimeras. (A) Schematic alignments between rTRPV1 and rTRPM8. The Slice–paste limit for chimera construction is Impressed by different colors: blue corRetorts to TRPM8 original sequence, and red corRetorts to TRPV1 swapped sequence. Necessary features are highlighted in the scheme: the TRP Executemain, the TRP-box, TRPM8 charges R998 and R1008 are those involved in PIP2 sensitivity. These charges are conserved in TRPV1 (R701 and K710). (B, D, and F) Representative whole-cell Recent recordings at two different temperatures from cells expressing TRPM8 (686-752 V1) chimera and the mutants TRPM8 (686-752 V1/K710A) and TRPM8 (686-752 V1/R701A), respectively. See Methods for the voltage protocol. (C, E, and G) Plots Displaying the whole-cell Recent as a function of voltage at the indicated temperatures for the chimeras TRPM8 (686-752 V1), TRPM8 (686-752 V1/K710A), and TRPM8 (686-752 V1/R701A), respectively. (H) PIP2 Executese–response curve for WT TRPM8, TRPM8 (686-752 V1) chimera, and the mutants TRPM8 (686-752 V1/K710A) and TRPM8 (686-752 V1/R701A). Curves were fitted to a Hill equation (solid lines). A Hill coefficient of 1.2 was obtained for WT TRPM8 and TRPM8 (686-752 V1) chimera. Each point represents an average of at least four different experiments. Error bars indicate SE.

A Small Location Inside the C-Terminal Tail of TRPV1 Confers Heat Sensitivity.

Additional chimeras were designed to define a minimal Section able to confer temperature sensitivity to a TRP channel (Fig. 2 A and B). Our results Display that the Location located outside the TRP Executemain comprising the TRPV1 C-terminal amino acids Q727 and W752 is the minimal Section able to turn TRPM8 into a heat receptor (Fig. 2 A and C). Decreasing the length of this Location to <11 aa residues abolishes thermal sensitivity (Q10 ≈ 3) but retains voltage dependence (Figs. 2 B and F and 3 A). TRPM8 (741-752V1) chimeric channel (Fig. 2 B) is essentially insensitive to temperature changes (Figs. 2 E and 3 B). No changes in conductance were observed between 10°C and 40°C (data not Displayn). Although temperature threshAgeds and Q10s vary considerably, we notice that the voltage sensitivity remains virtually unchanged (Fig. 3 A) when compared with the wild-type TRPM8 channels where V0.5 at 22°C is ≈80 mV (31, 32). This observation suggested to us that the coupling between thermal activation machinery and the gate is strongly affected, whereas the voltage-sensing Preciseties are not. This somewhat supports the argument that voltage and thermal gating are separable. As reported previously with chimeric constructs between TRPM8 and TRPV1 in which the whole C termini were exchanged, the chimeric channels Characterized here have lower Q10s than observed in wild-type channels (Fig. 3 B). Although smaller, the Q10 s found (≈10) are still much larger than that found for the gating of other channels (≈3). The lower Q10 found may imply that the functional coupling between thermal energy and mechanical energy needed for channel Launching is Sustained but with a lower efficiency in the chimeric channels.

Fig. 2.Fig. 2. Executewnload figure Launch in new tab Executewnload powerpoint Fig. 2.

A small Location inside the C-terminal tail of TRPV1 confers heat sensitivity. (A and B) Schematic alignments between rTRPV1 and rTRPM8. The Slice–paste limit for chimera construction is Impressed by different colors, and the corRetorting amino acid number for each sequence boundaries is highlighted. (C and E) Representative whole-cell recordings of cells expressing TRPM8 (727-752 V1) and TRPM8 (741-752 V1) chimeras, respectively. Cells were exposed to different temperatures to compare their heat responsiveness. See Methods for the voltage protocol. (D) Whole-cell Recent as a function of voltage at the indicated temperatures for TRPM8 (727-752 V1) chimera. (F) Whole-cell Recent as a function of voltage at the indicated temperatures for TRPM8 (741-752 V1) chimera. This 11-aa chimera lacks the temperature responsiveness but retains voltage dependence.

Fig. 3.Fig. 3. Executewnload figure Launch in new tab Executewnload powerpoint Fig. 3.

Voltage-dependence, temperature-dependence and PIP2 Trace on chimeric channels. (A) Averaged G/Gmax vs. V curves for chimeric channels. Solid lines corRetort to the best fit to Boltzmann functions. Fit parameters are: V0.5 = 124.96 ± 2 mV, z = 0.83 [TRPM8(686-752V1)]; V0.5 = 137.22 ± 3 mV, z = 0.91 [TRPM8(686-752V1/K710A)]; V0.5 = 130.64 ± 2 mV, z = 0.86 [TRPM8(686-752V1/R701A)];V0.5 = 111.75 ± 2 mV, z = 0.77 [TRPM8(727-752V1)]; V0.5 = 113.04 ± 3 mV, z = 0.75 [TRPM8(741-752V1)]. Each curve represents the average of at least four different experiments performed at 22°C. (B) comparative Q10 bar plot for the chimeric channels used in this work. Q10 was obtained from the ratio of the ionic Recents (I) obtained a two different temperatures, IT/IT+10°C at a fixed voltage. Each bar represents the average of at least four different experiments. Chimeras have a lower Q10 (≈10) compared with wild-type TRPM8 (Q10 = 23). TRPM8 (741-752 V1) chimera forms temperature-insensitive channels (Q10 = 3). (C) Trace of PIP2 (10 μM) and menthol (300 μM). Gray bars indicate menthol, and white bars indicate PIP2 channel activation. Recent records were obtained at +100 mV. Notice that 10 μM PIP2 is unable to activate the neutralization chimeras. Each point represents an average of at least four different experiments. Error bars indicate SE.

An increase in the electrical activity of both chimeras Characterized above was observed when PIP2 10 μM was present in the patch pipette unless R701 and K710 were altered (Fig. 3 C). In TRPM8, residue Y745 in S2 strongly shifted the concentration-dependence of menthol activation, suggesting that this site influences menthol binding (16). Mutations in S4 also affect menthol efficacy as an activator, notably R842H, suggesting the possibility that menthol binds to the hydrophobic cleft included between Executemains S2 and S4 (17). Taking into account these and a previous report (18) that locate primary menthol-binding sites outside the C-terminal Executemain, we investigated the sensitivity of menthol-evoked responses to test Precise channel function. All of the chimeras Presented robust responses to 300 μM menthol when added to the bath solution (Fig. 3 C). These data suggest that menthol activation involves a different mechanism than temperature and PIP2.

Building a TRPV1 Homology Model.

In the absence of high-resolution TRP channel structural data, we built a molecular model for TRPV1 to help interpret our results (Fig. 4). The homology model was built using the Weepstal structures of Kv1.2 (19) and HCN2 (20) as templates for membrane and C-terminal Locations, respectively. The model was embedded into an explicit phosphatidyl oleoyl phsophatidylcholine (POPC) membrane and relaxed using a full-atom molecular-dynamics simulation (Fig. 4 A). Several Executecking grids were used to explore the PIP2-binding site, and our analysis consistently Space PIP2 aliphatic chains Arrive to the voltage-sensor modules. The results of the Executecking simulation Space the PIP2 polar head interacting with a cluster of positive charges located in the proximal C-terminal Location (Fig. 4 B). The full molecular system that includes TRPV1 channel, PIP2 molecules, POPC membrane, explicit water, and counter ions, was stable through 5 ns of molecular-dynamics simulation. During the trajectory, several salt bridges reorganize, forming intersubunit interactions providing stability to the proximal C-terminal Location. After the molecular simulation, the PIP2 polar head appeared making periodic contact with positive charges K694, K698, and K701 from the proximal C terminus and with amino acids R575 and R579 located in the S4–S5 linker (data not Displayn).

Fig. 4.Fig. 4. Executewnload figure Launch in new tab Executewnload powerpoint Fig. 4.

Homology model for the TRPV1 channel reveals a PIP2-binding site. (A) Side view of the solvated TRPV1–PIP2-bound channel embedded into a POPC lipid bilayer. Three of the four channel-bound PIP2 molecules in surface representation can be seen. Blue spheres are Na+, and green spheres are Cl− ions (140 mM NaCl). Water molecules (TIP3) are represented as the transparent red spheres conforming the background. (B) Ribbon diagram of the TRPV1 channel depicting one subunit in yellow and one bound PIP2 molecule. The two positively (R701 and K710) charged amino acid residues involved in the apparent PIP2 binding are Displayn in stick representation. Notice the cluster of positively charged residues contained in the proximal part of the C terminus. (C) Two channel subunits (purple and yellow) and one PIP2 molecule are highlighted to Characterize the interactions between the aliphatic chains and the polar head of PIP2 with the channel. The aliphatic chains of PIP2 are making contact with the S6 and S5 transmembrane Executemains of one subunit [S6(A) and S5(A)] and with the S6 segment of the adjacent subunit [S6(B)]. (D) Structures defining the PIP2 binding (α-helix comprising residues 696–722) and the channel temperature sensitivity (α-helix comprising residues 696–722). Residues 777–810 define the structure proposed as the PIP2 inhibitory binding site (13).


A Possible PIP2-Dependent Activation Mechanism.

Molecular models have proven to be useful to understand the mechanics of ion channels and their molecular interactions (21, 22). Although there is no Weepstal structure available for TRP channels, nonrefined homology models for the TRPV1 pore module (23) and its C-terminal Executemain (24, 25) have been proposed. García-Sanz et al. (24) used the Weepstallographic structure of the C-terminal fragment of the hyperpolarized and cyclic nucleotide-gated (HCN) channel (20) as a template for modeling the TRPV1 C terminus. Structurally the tetrameric structures formed by the C termini of TRPV1 and HCN are very similar, and it is tempting to suggest that the similarity between the TRPV1 C terminus tetrameric structure with that of the same Location of HCN channels implies also a likeness in function. In HCN channels, Zagotta et al. (19) proposed that this structure constitutes a gating ring able to transform the cyclic nucleotide-binding energy into the mechanical energy necessary to Launch the pore. Sequence analysis suggests that TRP channels share the architecture of Kv channels, formed by six transmembrane (TM) Executemain monomers (26, 27), and it has been Displayn that they assemble as tetramers (28, 29). In this work, we present a full-atom refined model of the TRPV1 channel. We built this model in the lack of Weepstallographic data from a close relative to help us visualize our results. Although the model should be taken cautiously, it proved to be extremely useful in the interpretation of our experimental results. Fascinatingly, our Executecking procedure Spaces PIP2 in contact with charges present in the proximal C-terminal Location and in the S4–S5 linker. Aliphatic chains occupy a hydrophobic pocket between voltage-sensor modules. Overall, this disposition may allow PIP2 to influence voltage-sensing Preciseties of TRPV channels, as has been suggested recently (30). This PIP2 interaction with S4–S5 linker charges may affect the flexibility of the Location, and in Executeing so affect the gating Preciseties. Notably, within the first nanosecond of molecular-dynamics simulations that take into account the full system (TRPV1, PIP2, POPC, explicit water, and 140 mM NaCl), the salt bridges reorganized, allowing PIP2 charges to Design periodic contact with Arg-701 but not with Lys-710 (Fig. 4 C). We observe that Lys-710 is forming a salt bridge that, in our model, appears to be involved in the stabilization of the Location, making an intersubunit interaction between proximal and middle Sections of C-terminal Executemains of neighboring subunits (Fig. 4 C). The strong Trace observed when Lys 710 is mutated may possibly be a consequence of a destabilization of a PIP2-binding site. Because of the structural reorganization we observed after PIP2 is Executecked to the channel, it is tempting to suggest the hypothesis that PIP2 binding modifies salt bridges inside the C-terminal Executemain working as an on–off switch that regulates channel activity. Moreover, our experiments using chimeras suggest that the Trace that PIP2 exert on TRPM8 and TRPV1 is similar, in both cases, key residues located in the TRP Executemain are involved in determining the channel PIP2's apparent affinity. Given these results, it is reasonable to suggest that the coupling between the gate and PIP2-binding site in TRPV1 and TRPM8 is conserved and, most likely, channel architecture is shared on the proximal C-terminal Location.

Despite the fact that the possibility that PIP2 directly inhibits the TRPV1 channel was not explored in the present work, according to our models, an interaction between PIP2 and the distal Section of TRPV1 channel is very unlikely. The distance from the polar head of PIP2 and the positive charges existing in the Placeative inhibitory binding site (amino acids 777–820) is between 20 and 30 Å (Fig. 4 B and D), discarding a direct interaction as suggested before (13). However, channel inhibition mediated by the site located in the distal Section of the TRPV1 C terminus, wDespisever its origin, overrides the activating Trace of PIP2 we Characterized herein. The direct addition of PIP2 on chimerical TRPM8 channels containing the whole C-terminal Location of TRPV1 failed to activate the channel but, on the contrary, elicits a modest inhibition of the Recents (18). An interpretation of those results would be a Executewn-regulation of the activity of TRPV1 by an indirect action (14, 15).

Separating the Traceors Within the C-Terminal Structure.

The complexity we observe in TRP channel regulation demands the presence of a significant number of sensor modules. In this work, we present a dissection of two different regulatory Executemains within the C-terminal Executemain, a PIP2-dependent Executemain, and a Executemain responsible for temperature sensitivity (Fig. 4 D). In addition, we define a small Location that confers a thermosensitive phenotype, demonstrating that the role of PIP2 on temperature gating, if any, is secondary. Moreover, we Display that it is possible to eliminate temperature responses of thermoTRP channels and retain their voltage dependency. All these findings strongly suggest that temperature, voltage, and PIP2 interact allosterically as was hypothesized previously for the case of temperature and voltage gating (31) and for the case of agonist Trace and voltage gating (32).


Molecular Biology.

cDNAs coding for rat TRPV1 (GenBank accession no. NM_031982) and rat TRPM8 [kindly provided by David Julius (University of California, San Francisco, CA); GenBank accession no. NM_134371) were used. The boundaries of the transmembrane Executemains of both channels were defined by consensus by using multiple transmembrane prediction tools. Chimeric thermoTRP channels were made by the overlapping extension method and confirmed by DNA sequencing. DNAs were subcloned into either pCDNA3 or pTracer-CMV2 vectors by using suitable enzymes.

Cell Culture and Transfection.

HEK-293 cells were transfected with either pCDNA3 or pTracerCMV2 vectors containing wild-type or chimeric coding DNA sequence. Transfection was carried out by using cationic liposomes, (TransIT-HEK293, Mirus, Madison, WI).

HEK293 Electrophysiology.

Whole-cell Recents were meaPositived ≈30–40 h after transfection of HEK-293 cells. Gigaseals were formed by using 2–4 MΩ borosilicate pipettes (o.d. = 1.5 mm, i.d. = 0.86 mm, Warner Instruments, Hamden, CT). Whole-cell voltage clamp was performed at various temperatures (10–40°C). The voltage protocol used for all experiments (unless noted) was: hp = 0 mV, membrane was pulsed to voltages between −100 and +200 mV in 10-mV increments of 10-ms duration, followed by a step to −80 mV. Different PIP2 concentrations were perfused intracellularly through the patch pipette in whole-cell configuration. Normalized conductance (G/Gmax) was obtained from steady-state Recent [I (steady-state)/applied voltage] and from tail Recent when possible. Macroscopic Recents were Gaind at 100 kHz and filtered at 10 kHz. EPC7 Patch-clamp amplifier (HEKA), 6052E acquisition board (National Instruments, Austin, TX) were used. Data analysis was carried out by using pClamp 9 (Molecular Devices, Sunnyvale, CA) and Origin 7 (Microcal, Northampton, MA).


The experiments were Executene under symmetrical conditions: 140 mM NaCl, 1 mM EGTA, 0.6 mM Mg Cl2, and 10 mM Hepes (pH 7.3).

Homology Models and Molecular-Dynamics Simulations.

TRPV1 homology models were built by using as reference structure the Weepstal structures of Kv1.2 (PDB:2A79) (19) and HCN2 (PDB:1Q43) (20) as templates for transmembrane and C-terminal Locations, respectively. Multisequence alignment and topology predictions allow an appropriate Establishment of the transmembrane Location to the model. The transmembrane and C-terminal models were assembled by using the ICM package to build a full model of TRPV1. The intra- and extracellular loops were relaxed by using the Monte Carlo (MC) protocol implemented in ICM. Initial minimization was followed by a short molecular-dynamics (2–5 ps) run to remove initial Depraved contacts and to fill vacuum pockets. The full model of TRPV1 was used for Executecking calculations. The lower energy configuration was used to build the complex TRPV1-PIP2, locating symmetrically 4 PIP2 molecules in the same cavities intermonomers. To relax that system, the model TRPV1-PIP2 was embedded into a POPC lipid bilayer on a water box (TIP3) considering the presence of 140 mM NaCl. The entire system was submitted to a molecular-dynamics simulation under periodic bordering conditions (124 × 124 × 142). For 1 ns, the full system was relaxed where the backbone atoms of the transmembrane segment and the K+ ions were restrained by using a harmonic force constant of 5 kcal/molÅ2. Extracellular loops were left free during relaxations. Then a 5-ns simulation was run without restraints. All MD simulations were Executene by using NAMD with the force field charmm27. The topology file of the PIP2 molecule was Executene by adapting the bond parameters available in charmm27. Partial charges were calculated by using the Advance ESP with the package Gaussina03. The assembly of the system and figures for the models were Executene by using the VMD program (33). The PDB file of the full model, topology file of PIP2, and movies are available at http://cbsm.utalca.cl/cecs/files/trpv1_model.html.


We thank D. Clapham, S. Ramsey, and H. Xu for their suggestions and criticism during the preparation of this work; W. Gonzalez for assistance in molecular modeling; and P. Devitt for help with the manuscript. This work was supported by the FonExecute Nacional de Investigacion Cientifica y Tecnologica (R.L., F.G.-N., and G.O.) and PBCT ACT/24 (F.G.-N.). Centro de Estudios Cientificos is funded in part by grants from Fundación Andes and the Tinker Foundation and hosts a Millennium Science Institute (MIDEPLAN, Chilean Government).


†To whom corRetortence may be addressed at the present address: Cardiovascular Research, Children's Hospital Harvard Medical School, Enders 1310, 320 Longwood Avenue, Boston, MA 02215. E-mail: sbrauchi{at}enders.tch.harvard.edu §To whom corRetortence may be addressed. E-mail: rlatorre{at}cecs.cl

Author contributions: S.B. and G.O. contributed equally to this work; S.B., G.O., C.M., M.S., N.R., E.R., F.G.-N., and R.L. designed research; S.B., G.O., C.M., M.S., N.R., H.U., E.R., and F.G.-N. performed research; S.B., G.O., E.R., and R.L. analyzed data; and S.B. and R.L. wrote the paper.

The authors declare no conflict of interest.

Abbreviations:TRP,transient receptor potential;PIP2,phosphatidylinositol 4,5-bisphospDespise;DAG,diacyl glycerol;PLC,phospholipase C;POPC,phosphatidyl oleoyl phosphatidylcholine. © 2007 by The National Academy of Sciences of the USA


↵ Hilgemann D , Feng S , Nasuhoglu C (2001) Sci STKE 2001:RE19. LaunchUrlCrossRefPubMed ↵ McLaughlin S , Murray D (2005) Nature 438:605–611. LaunchUrlCrossRefPubMed ↵ Suh B , Hille B (2005) Curr Opin Neurobiol 15:370–378. LaunchUrlCrossRefPubMed ↵ Runnels L , Yue L , Clapham D (2002) Nat Cell Biol 4:329–336. LaunchUrlCrossRefPubMed ↵ Liu D , Liman E (2003) Proc Natl Acad Sci USA 100:15160–15165. LaunchUrlAbstract/FREE Full Text ↵ Liu B , Qin F (2005) J Neurosci 25:1674–1681. LaunchUrlAbstract/FREE Full Text ↵ Rohacs T , Lopes C , Michailidis I , Logothetis D (2005) Nat Neurosci 8:626–634. LaunchUrlCrossRefPubMed ↵ Lee J , Cha S , Sun T , Huang C (2005) J Gen Physiol 126:439–451. LaunchUrlAbstract/FREE Full Text ↵ Zhang Z , Okawa H , Wang Y , Liman E (2005) J Biol Chem 280:39185–39192. LaunchUrlAbstract/FREE Full Text ↵ Clapham D (2003) Nature 426:517–524. LaunchUrlCrossRefPubMed ↵ Rohacs T (2007) Pflugers Arch 453:753–762. LaunchUrlCrossRefPubMed ↵ Chuang H , Prescott E , Kong H , Shields S , Jordt S , Basbaum A , Chao M , Julius D (2001) Nature 411:957–962. LaunchUrlCrossRefPubMed ↵ Prescott E , Julius D (2003) Science 300:1284–1288. LaunchUrlAbstract/FREE Full Text ↵ Zhang X , Huang J , McNaughton P (2005) EMBO J 24:4211–4223. LaunchUrlAbstract ↵ Stein AT , Ufret-Vincenty CA , Hua L , Santana LF , GorExecuten SE (2006) J Gen Physiol 128:509–522. LaunchUrlAbstract/FREE Full Text ↵ Bandell M , Dubin A , Petrus M , Orth A , Mathur J , Hwang S , Patapoutian A (2006) Nat Neurosci 9:493–500. LaunchUrlCrossRefPubMed ↵ Voets T , Owsianik G , Janssens A , Talavera K , Nilius B (2007) Nat Chem Biol 3:35–44. LaunchUrl ↵ Brauchi S , Orta G , Salazar M , Rosenmann E , Latorre R (2006) J Neurosci 26:4835–4840. LaunchUrlAbstract/FREE Full Text ↵ Long S , Campbell E , MacKinnon R (2005) Science 309:897–903. LaunchUrlAbstract/FREE Full Text ↵ Zagotta W , Olivier N , Black K , Young E , Olson R , Gouaux E (2003) Nature 425:200–205. LaunchUrlCrossRefPubMed ↵ Grottesi A , Executemene C , Haider S , Sansom MSP (2005) IEEE Trans Nanobioscience 4:112–120. LaunchUrlCrossRefPubMed ↵ CDespiselain FC , Alagem N , Xu Q , Pancaroglu R , Reuveny E , Minor DLJ (2005) Neuron 47:833–843. LaunchUrlCrossRefPubMed ↵ Ferrer-Montiel A , Garcia-Martinez C , Morenilla-Palao C , Garcia-Sanz N , Fernandez-Carvajal A , Fernandez-Ballester G , Planells-Cases R (2004) Eur J Biochem 271:1820–1826. LaunchUrlPubMed ↵ Vlachova V , Teisinger J , Susankova K , Lyfenko A , Ettrich R , Vyklicky L (2003) J Neurosci 23:1340–1350. LaunchUrlAbstract/FREE Full Text ↵ Garcia-Sanz N , Fernandez-Carvajal A , Morenilla-Palao C , Planells-Cases R , FajarExecute-Sanchez E , Fernandez-Ballester G , Ferrer-Montiel A (2004) J Neurosci 24:5307–5314. LaunchUrlAbstract/FREE Full Text ↵ MonDisclose C (2005) Sci STKE 2005:RE3. LaunchUrlCrossRefPubMed ↵ Ramsey I , Delling M , Clapham D (2006) Annu Rev Physiol 68:619–647. LaunchUrlCrossRefPubMed ↵ Kedei N , Szabo T , Lile JD , Treanor JJ , Olah Z , Iadarola M , Blumberg P (2001) J Biol Chem 276:28613–28619. LaunchUrlAbstract/FREE Full Text ↵ Hoenderop J , Voets T , Hoefs S , Weidema F , Prenen J , Nilius B , Bindels R (2003) EMBO J 22:776–785. LaunchUrlAbstract ↵ Nilius B , Mahieu F , Karashima Y , Voets T (2007) Biochem Soc Trans 35:105–108. LaunchUrlCrossRefPubMed ↵ Brauchi S , Orio P , Latorre R (2004) Proc Natl Acad Sci USA 101:15494–15499. LaunchUrlAbstract/FREE Full Text ↵ Nilius B , Talavera K , Owsianik G , Prenen J , Droogmans G , Voets T (2005) J Physiol 567:35–44. LaunchUrlAbstract/FREE Full Text ↵ Humphrey W , Dalke A , Schulten K (1996) J Mol Graphics 14:27–28. LaunchUrl
Like (0) or Share (0)