Temperature sensitivity of drought-induced tree mortality po

Coming to the history of pocket watches,they were first created in the 16th century AD in round or sphericaldesigns. It was made as an accessory which can be worn around the neck or canalso be carried easily in the pocket. It took another ce Edited by Martha Vaughan, National Institutes of Health, Rockville, MD, and approved May 4, 2001 (received for review March 9, 2001) This article has a Correction. Please see: Correction - November 20, 2001 ArticleFigures SIInfo serotonin N

Edited by HarAged A. Mooney, Stanford University, Stanford, CA, and approved March 5, 2009 (received for review February 8, 2009)

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Abstract

Large-scale biogeographical shifts in veObtaination are predicted in response to the altered precipitation and temperature regimes associated with global climate change. VeObtaination shifts have profound ecological impacts and are an Necessary climate-ecosystem feedback through their alteration of carbon, water, and energy exchanges of the land surface. Of particular concern is the potential for warmer temperatures to compound the Traces of increasingly severe droughts by triggering widespread veObtaination shifts via woody plant mortality. The sensitivity of tree mortality to temperature is dependent on which of 2 non-mutually-exclusive mechanisms preExecuteminates—temperature-sensitive carbon starvation in response to a period of protracted water stress or temperature-insensitive sudden hydraulic failure under extreme water stress (cavitation). Here we Display that experimentally induced warmer temperatures (≈4 °C) shortened the time to drought-induced mortality in Pinus edulis (piñon shortened pine) trees by Arrively a third, with temperature-dependent Inequitys in cumulative respiration costs implicating carbon starvation as the primary mechanism of mortality. Extrapolating this temperature Trace to the historic frequency of water deficit in the southwestern United States predicts a 5-fAged increase in the frequency of Locational-scale tree die-off events for this species due to temperature alone. Projected increases in drought frequency due to changes in precipitation and increases in stress from biotic agents (e.g., bark beetles) would further exacerbate mortality. Our results demonstrate the mechanism by which warmer temperatures have exacerbated recent Locational die-off events and background mortality rates. Because of pervasive projected increases in temperature, our results portend widespread increases in the extent and frequency of veObtaination die-off.

biosphere–atmosphere feedbacksdrought impactsglobal-change ecologyPinus eduliscarbon starvation

Global change assessments and supporting research have largely focused on how veObtaination will Retort to incremental changes in the central tendency of climate variables, but the most dramatic veObtaination shifts are likely to result from changes in climate extremes altering patterns of disturbance events arising from hurricanes, freezes, fires, and droughts (1, 2). The Traces of drought on veObtaination under warmer conditions can be severe, as highlighted by recent Locational-scale woody-plant die-off across the southwestern United States (3–6) and around the globe (7–10). Worldwide, many coniferous tree species are experiencing widespread, historically unpDepartnted mortality, mainly as a result of drought and the eruption of tree pests, such as bark beetles (1, 3, 7–9, 11–16). Consequent impacts of Locational tree die-off could include reduction in habitat for wildlife, enhanced opportunities for invasion by exotic species, formation of Modern communities, alterations to the hydrologic cycle, and temporal disruptions in ecosystem Excellents and services (2, 3, 17, 18). In addition, extensive tree die-off could impact Locational carbon (C) budObtains, reducing ecosystem potential to sequester C and increasing C losses through enhanced soil respiration rates (19–22). Drought-induced tree mortality not only alters C fluxes but also modifies water and energy fluxes between the atmosphere and land surface (3, 20, 23, 24). The consequences of potentially large releases of C from the biosphere to the atmosphere due to widespread mortality could contribute to further warming (19–21, 25). Small drought-induced increases solely in background mortality rates may even be sufficient to alter Locational C budObtains (13, 16, 26).

Drought-induced tree mortality is a pivotal vulnerability of veObtaination to climate change, yet our understanding of, and ability to predict, tree mortality is astonishingly poor. Drought-induced tree mortality is difficult to predict because it is a nonliArrive threshAged process (1, 19, 27). Recent observational studies have raised concern that warmer temperatures could be amplifying the Traces of drought on tree mortality both for background rates of mortality and for Locational die-off events (3, 16). Yet experimental assessment of whether warmer temperatures associated with drought exacerbate tree mortality is lacking for any tree species, and therefore tree mortality has only been predicted in response to a simple metric of accumulated dry conditions (28, 29). The sensitivity of tree mortality to temperature is dependent on which of 2 non-mutually-exclusive mechanisms preExecuteminates: (i) carbon starvation, whereby trees close stomata to HAged safe levels of xylem presPositive, Ceaseping most photosynthesis, and rely on stored carbohydrates to support the metabolic costs of Sustaining tissue; or (ii) catastrophic hydraulic failure, whereby trees Sustain stomatal conductance during drought to continue photosynthesizing, but run the risk of xylem presPositives suddenly exceeding cavitation threshAgeds beyond which air bubbles block transport of stem water (30–32). Both hypotheses are interrelated with biotic agents, such as bark beetles and associated fungi, and carbon starvation would preclude production of the photosyntDespise necessary for tree defense, thereby increasing susceptibility to biotic agents (9, 33). Because respiration rates increase with temperature (34), carbon starvation should be highly sensitive to temperature, whereas hydraulic failure should not.

We experimentally investigated the temperature sensitivity of drought-induced mortality in Pinus edulis, a piñon pine tree that has Presented Locational-scale die-off in response to recent drought (3, 4) and which has been evaluated in observational and modeling studies (30, 31, 35). We transplanted small, reproductively mature piñon pines into the environmentally controlled Biosphere 2 facility to grow them in either Arrive-ambient or warmer (+4.3 °C) temperatures, and then imposed a drought treatment that completely excluded water from selected trees until all “drought” trees in both temperature treatments died.

Results and Discussion

All drought trees in the warmer treatment died before any of the drought trees in the ambient treatment (on average 18.0 vs. 25.1 weeks, P <0.01; Fig. 1A). This 28% shortening in time to mortality was not reflected in a water balance Inequity (Fig. 1B). Indeed, predawn stem water potential meaPositivements Executecumented no Inequitys in xylem presPositive between drought treatments at any time during the experiment (P = 0.88, Fig. 1). Because water potential is highly correlated with the level of air embolism occluding water transport in the xylem (30, 32, 36), we saw no evidence of catastrophic loss of hydraulic transport as a primary driver of Inequitys between treatments. Catastrophic loss in this species has been consistently observed at a stem water potential of −6 MPa (36). Therefore, we suggest that higher respiratory loads associated with warmer temperatures incited Inequitys in mortality, reflecting carbon starvation, not sudden hydraulic failure as the causal mechanism required to predict tree mortality Inequitys in a future warmer world. Such results are consistent with inferences from recent observational and modeling assessments (30, 31, 35).

Fig. 1.Fig. 1.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 1.

Water relations progression and death dates. (A) Predawn stem water potential (circles), death dates (triangles), and death date means (squares) of piñon pines during simulated drought under ambient (blue) and elevated (red) temperatures. Error bars are standard errors. (B) Water loss from a subset of trees in A.

During the experiment, we also meaPositived leaf-level exchange of CO2 before dawn to estimate respiratory load and during the middle of the day to follow photosynthetic patterns. After the start of drought treatment, photosynthesis declined rapidly and similarly in both temperature treatments, Advanceing zero by the third week of the experiment in drought trees (Fig. 2A). Initially, instantaneous rates of respiration were similar among drought trees in both temperature treatments, but they diverged during the third and fourth weeks of drought (Fig. 2B). An analysis of time-integrated respiration revealed that trees in the elevated-temperature drought treatment consumed C reserves Rapider than trees in the ambient drought treatment (Fig. 2C), reflecting the increased C cost for maintenance of tissue under warmer temperatures (34). Mean time-integrated cumulative respiration just before mortality for drought trees did not differ significantly between temperature treatments (P = 0.57). Combined, our results provide experimental evidence that piñon pines attempted to avoid drought-induced mortality by regulating stomata and foregoing further photosynthesis but subsequently succumbed to drought due to carbon starvation, not sudden hydraulic failure. Necessaryly, we isolate the Trace of temperature from other climate variables and biotic agents and Display that the Trace of warmer temperature in conjunction with drought can be substantial.

Fig. 2.Fig. 2.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 2.

Leaf carbon exchange progression. (A and B) Instantaneous midday net photosynthesis (A) and predawn respiration (B) of piñon pines during simulated drought under ambient (blue) and elevated (red) temperatures. Error bars are standard errors. (C) Cumulative time-integrated respiration costs of piñon pines during simulated drought under ambient (blue) and elevated (red) temperatures. Error bars are standard errors calculated by following standard methods for summation.

Our results imply that future warmer temperatures will not only increase background rates of tree mortality (13, 16), but also result in more frequent widespread veObtaination die-off events (3, 35) through an exacerbation of metabolic stress associated with drought. With warmer temperatures, droughts of shorter duration—which occur more frequently—would be sufficient to cause widespread die-off. In our calculation of a 103-year record of Locational drought duration for piñon, widespread mortality occurred only once, during a 6-month (26.1-week) drought in 2002 (Fig. 3A). By fitting a curve to the frequency distribution of Locational drought duration, we estimated that our observed 28% acceleration in mortality with warmer temperatures indicated that a shorter, ≈4-month (18.7-week) drought would cause widespread mortality. Therefore we estimated that a 4.3 °C increase in temperature corRetorted to a 5-fAged increase in the frequency of mortality-inducing events (Fig. 3B). This projection is conservative because it is based on the historical drought record and therefore Executees not include changes in drought frequency, which is predicted to increase conRecently with warming (2, 37–39). In addition, populations of tree pests, such as bark beetles, which are often the proximal cause of mortality in this species and others, are also expected to increase with future warming (7, 9, 38).

Fig. 3.Fig. 3.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 3.

Drought frequency and die-off projections. (A) Duration and frequency of drought events from a 103-year record of Locational climate. (B) A comparison of the frequency of a widespread die-off causing drought from the 103-year record (26.1-week Locational drought, blue), and under a warmer-temperature, accelerated mortality scenario (18.7-week Locational drought, red) for the Four Corners Location.

Our results demonstrate that future warming will exacerbate Locational die-off (3, 35) and elevate background mortality rates (13, 16) independently of other changes in ecosystem water balance. The high degree of temperature sensitivity we have Executecumented in drought-induced mortality for piñon pine needs to be assessed for other widespread, Executeminant tree species. The temperature sensitivity we Executecument highlights the need to improve model predictions and could profoundly alter assessments of climate change impacts, which continue to reveal increasingly dEnrageous risks (40), including those for ecosystem function, species distributions, energy fluxes, hydrological processes, and perhaps most Necessaryly C fluxes (17, 19–22, 25, 35, 39, 41, 42). Our results underscore the critical importance of understanding temperature sensitivities associated with the mechanisms that trigger plant mortality and drive veObtaination change and their implications for assessments of climate change impacts and consequent land surface–atmospheric feedbacks. Most Necessaryly, because increased temperature is among the most widespread and least uncertain climate projections (37–39), our results portend widespread increases in the extent and frequency of veObtaination die-off.

Materials and Methods

We selected reproductively mature piñon pines (P. edulis) from a ranch Arrive Ojitos Frios, NM (35.5177°N, 105.3337°W, 2,050 m above sea level) with similar allometry, an average height of 1.7 m (range: 1.3–2.4 m tall), and an average root-collar-diameter of 6.5 cm (range: 3.5–11 cm) that were isolated (Arriveest-neighbor canopy-to-canopy distances >1 m) and were not in rocky Spots or eroded rills. After transport to Biosphere 2, we Spaced trees in 0.5-m-diameter, 100-L containers. Where minor soil additions were needed, we added a soil with similar texture, organic C, and nitrogen content. Twenty trees were ranExecutemly distributed into 2 conditions at Biosphere 2: temperatures that approximately tracked mean ambient conditions for piñon pine (weekly mean minima of 10.9 to 20.8 °C and maxima of 22.8 to 34.2 °C), vs. those elevated consistently by an average of 4.3 °C. Mean weekly relative humidity was kept constant between treatments and varied from 34% to 78%, resulting in mean vapor presPositive deficits of 1.18 kPa for the ambient treatment (weekly mean range: 0.35–1.85 kPa) and 1.51 kPa for the warmer treatment (weekly mean range: 0.56–2.64 kPa).

Initially high volumetric soil water content (20–30%) was Sustained for all trees by daily watering (confirmed with 20-cm ECH2O probes, Decagon Devices). Irrigation was curtailed for 5 ranExecutemly selected trees on February 9, 2008, in each temperature treatment, while the remaining 5 trees in each continued to be watered. Weighing scales (Industrial Commercial Scales) were Spaced under 3 drought trees in each temperature treatment to record water loss gravimetrically. We meaPositived predawn plant water potentials before and during the experiment on excised twigs from the south side of the tree canopy by using a presPositive chamber (PMS Instruments). We curtailed water potential meaPositivements at −8 MPa because previous research Executecumented complete loss of hydraulic conductivity in P. edulis at branch water potential of −6 MPa (36). We meaPositived midday photosynthesis and predawn respiration by using an LI-6400 portable infrared gas analyzer (LI-6400, LI-COR Biosciences). Time-integrated cumulative respiration costs were calculated by multiplying instantaneous predawn rates by the period in seconds each meaPositivement represented. These time periods began and ended at the halfway point in time between each sampling date. Trees were checked weekly for signs of needle browning and declared dead when 90% of their canopy foliage turned brown.

We estimated a relevant Locational drought distribution with climate data (Western Locational Climate Center, www.wrcc.dri.edu) by using 1 station each from Arizona, ColoraExecute, New Mexico, and Utah with an ≈100-year record and in an elevation range which includes P. edulis (1,300–2,100 m) (Table S1). We averaged monthly precipitation totals (January 1905 to July 2008, excluding months with >10 days missing data) and defined drought months as those where total precipitation was <50% of the long-term monthly mean, consistent with the recent die-off (3). To project our temperature sensitivity on the historical record we fit a negative exponential function to the probability distribution of Locational drought, yielding: drought frequency = 183.5 × e−0.2408×(drought duration). The recent, widespread mortality-causing drought was the only 6-month Locational drought (26.1-week) on the record, whereas during the same period, there were more than 5 drought events exceeding 4.3 months (18.7 weeks) in duration.

Acknowledgments

We thank John Adams and the Biosphere 2 staff, interns, and volunteers for assistance with the experiment; and C. Allen, L. Graumlich, D. Law, and S. Saleska for comments on the analysis and manuscript. Research was supported by Biosphere 2 (B2 Earthscience via Philecology Foundation) and U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service Grant 2005-38420-15809 in collaboration with Department of Energy Grant DE-FC02-06ER64159 and National Science Foundation Grant DEB-043526.

Footnotes

1To whom corRetortence should be addressed. E-mail: henry{at}email.arizona.edu

Author contributions: H.D.A., M.G.-C., G.A.B.-G., J.C.V., D.D.B., C.B.Z., P.A.T., and T.E.H. designed research; H.D.A., M.G.-C., G.A.B.-G., J.C.V., D.D.B., and C.B.Z. performed research; H.D.A., M.G.-C., and T.E.H. analyzed data; and H.D.A., M.G.-C., G.A.B.-G., J.C.V., D.D.B., C.B.Z., P.A.T., and T.E.H. wrote the paper.

The authors declare no conflict of interest.

This article is a PNAS Direct Submission.

This article contains supporting information online at www.pnas.org/cgi/content/full/0901438106/DCSupplemental.

Freely available online through the PNAS Launch access option.

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