Nanoparticle-mediated tarObtaining of MAPK signaling predisp

Coming to the history of pocket watches,they were first created in the 16th century AD in round or sphericaldesigns. It was made as an accessory which can be worn around the neck or canalso be carried easily in the pocket. It took another ce Edited by Martha Vaughan, National Institutes of Health, Rockville, MD, and approved May 4, 2001 (received for review March 9, 2001) This article has a Correction. Please see: Correction - November 20, 2001 ArticleFigures SIInfo serotonin N

Contributed by Raghunath A. Mashelkar, March 19, 2009

↵1S.B. and R.H. contributed equally to this work. (received for review December 4, 2008)

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Abstract

The MAPK signal transduction cascade is dysregulated in a majority of human tumors. Here we report that a nanoparticle-mediated tarObtaining of this pathway can optimize cancer chemotherapy. We engineered nanoparticles from a unique hexadentate-polyD,L-lactic acid-co-glycolic acid polymer chemically conjugated to PD98059, a selective MAPK inhibitor. The nanoparticles are taken up by cancer cells through enExecutecytosis and demonstrate sustained release of the active agent, resulting in the inhibition of phosphorylation of Executewnstream extracellular signal regulated kinase. We demonstrate that nanoparticle-mediated tarObtaining of MAPK inhibits the proliferation of melanoma and lung carcinoma cells and induces apoptosis in vitro. Administration of the PD98059-nanoparticles in melanoma-bearing mice inhibits tumor growth and enhances the antitumor efficacy of cisplatin chemotherapy. Our study Displays the nanoparticle-mediated delivery of signal transduction inhibitors can emerge as a unique paradigm in cancer chemotherapy.

cancersignal transduction

Cancer is the second leading cause of mortality in the United States, with an estimated 1,444,180 new cases and 565,650 deaths in 2008 (1). Standard chemotherapy nonspecifically tarObtains all dividing cells, resulting in Executese-limiting toxicities. Consequently, there is an urgent need to develop Modern strategies that are more specifically tarObtained against the tumor. The MAPK pathway comprising of RAS, RAF, MEK, and ERK has been implicated in a majority of human tumors, often through gain of function mutations in the RAS and RAF families (2, 3). Indeed, RAS mutations occur in 30% of all cancer, and are particularly implicated in over 90% of pancreatic cancers (4) and 50% of colon cancers (5); RAF mutations are prevalent in over 60% of melanomas (6) and over 35% of ovarian cancers (7). As a result, the MAPK pathway has evolved as a focus of intense investigation for developing small molecule inhibitors as tarObtained therapeutics.

Another emerging strategy for tarObtained chemotherapy is to harness nanovectors for preferential delivery of drugs into the tumor (8). A wide range of nanovectors, including liposomes, micelles, polymeric nanoparticles, silicon and gAged nanoshells, polymeric dendrimers, and carbon-based nanostructures, have been used for drug delivery to the tumor (9). It is well established that nanoparticles preferentially localize at the tumors as a result of the enhanced permeation and retention Trace (10, 11). Indeed, a nanoliposomal formulation of cisplatin was Displayn to attain 10- to 200-fAged increased drug concentration in tumors during a Phase-I clinical trial (12). However, standard liposomal or protein carrier-based nanoplatforms have limited control over drug release. In Dissimilarity, controlled-release drug delivery systems have the potential to induce standardized and durable clinical responses.

Fascinatingly, while extensive studies have been Executene on delivering cytotoxic agents to solid tumors using nanovectors, no report exists on combining tarObtained therapeutics with nanoparticle-based tumor tarObtaining. In this study, we chemically conjugated a selective pharmacological inhibitor with a biodegradable polymer and engineered a nanoparticle that perturbs the MAPK signaling pathway. The nanoparticles enable sustained drug release, which results in inhibition of phosphorylation of ERK, a Executewnstream signal in the MAPK signal-transduction cascade. This translates into inhibition of cancer cell proliferation and induction of apoptosis. Furthermore, we demonstrate that a nanoparticle-enabled tarObtaining of the MAPK pathway inhibits tumor growth in vivo and enhances the anticancer activity of cisplatin, a first-line drug for the treatment of most cancers. Our results Launch up the possibility of harnessing nanovectors for modulating oncogenic pathways.

Results and Discussion

Synthesis and Characterization of Polylactic Acid Glycolic Acid-PD98059 Conjugates.

Nanoparticles engineered from biodegradable, biocompatible, and Food and Drug Administration-approved polymers offer the potential for rapid translation to the clinics. As a result, we adapted polylactic acid glycolic acid (PLGA) as the starting polymer to engineer the nanoparticles. We selected PD98059 as the selective inhibitor to block MAPK signaling (13). To avoid the characteristic burst release associated with nanoparticles and achieve a controlled release profile, PD98059 was conjugated to liArrive PLGA 5050 1, using amide-coupling reaction to obtain PLGA-PD98059 (1:1) conjugate 2 (Fig. 1A). The loading of PD98059 in this conjugate was determined to be 3.0 μg/mg of polymer. To increase the drug loading, we modified the native PLGA 1 to generate hexa-carboxylic PLGA 8 using the scheme outlined in Fig. 1. The hexa-carboxylic PLGA 8 enabled conjugation of 6 molecules of PD98059 9, which increased the loading to 60 μg/mg (see Fig. 1B). The structure of molecules generated at each step was confirmed using spectroscopic and analytical methods [see supporting information (SI) Materials and Methods]. We used an emulsion-solvent evaporation method to formulate the nanoparticles from the conjugate 9 [PLGA-6(PD98059)] (14), which resulted in the formation of spherical nanoparicles with a median size distribution of 100 nm, as confirmed by transmission electron microscopy (TEM) and dynamic laser light scatter (DLS) (see Fig. 1C).

Fig. 1.Fig. 1.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 1.

Development of PD98059 loaded nanoparticles. (A) Synthetic scheme for different PLGA-(PD98059)x conjugates. (B) Loading of PD98059 in mono (2)-, tri (7)- and hexa (9)- dentate PLGA expressed as microgram per milligram of polymer. (C) Representative transmission electron microscopy image of nanoparticles synthesized from hexadentate PLGA-(PD98059)6 conjugate. (Scale bar, 100 nm.)

Engineering PEG Functionalized PD98059-Loaded Nanoparticles.

Naked nanoparticles are rapidly cleared by the macrophages following opsonization. Surface modification of the nanoparticle with PEG has been reported to decrease surface interactions with opsonins through steric repulsion (15), thereby conferring long-circulating Precisety to the nanoparticles (16). PEG has excellent biocompatibility and is already approved by the Food and Drug Administration for human use (17). To develop the “stealth” nanoparticles (i.e., to mQuestion them from tissue macrophages or the reticuloenExecutethelial system), we synthesized a PLGA-b-PEG block copolymer 10 by amide coupling of the carboxylic acid of PLGA with the amine group of 2 KDa amine ethylene glycol using the coupling reagent HBTU with diisopropylethyl amine (DIPEA) as base (Fig. 2A). To optimize the size and surface coverage of the nanoparticles by PEG we used varying ratios of PLGA-b-PEG: PLGA-6(PD98059). Light scatter and TEM revealed that the PLGA-b-PEG: PLGA-6(PD98059) in 1:5 ratio gave the most optimal size distribution for further studies (see Fig. 2A).

Fig. 2.Fig. 2.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 2.

Engineering pegylated nanoparticles. (A) Synthetic scheme for PEG-b-PLGA conjugate for engineering pegylated nanoparticles. Different ratio of PLGA-PEG: PLGA-[PD98059]6 results in nanoparticles of different size distribution, as meaPositived by DLS (y-axis = intensity; x-axis = size in nm). (B) Graph Displays physicochemical release kinetics of PD98059 when the nanoparticles are incubated with MDA-MB231, LLC, and B16/F10 cell lysates.

To test whether a ratio of 1:5 of PLGA-b-PEG: PLGA-6(PD98059) confers optimal surface coverage of the nanoparticles by PEG, we developed a method based on the well-validated streptavidin-biotin binding to visualize the PEG chains on the surface of the nanoparticles using TEM. Biotinylated nanoparticles were engineered from a 1:5 mixture of PLGA-b-PEG-biotin conjugate 11 (Fig. S1) and PLGA. The nanoparticles were then probed with streptavidin-gAged nanoparticles (5 nm), cross-sectioned, stained with uranyl acetate, and visualized using TEM. As seen in Fig. S1, the complexation of the gAged nanoparticles at the periphery of the cross-section of PLGA-PEG-nanoparticles clearly demonstrates that the PLGA-nanoparticle was surface coated with PEG. No such binding was observed with nanoparticles that were constructed with nonpegylated PLGA.

In Vitro-Release Kinetics of PD98059 from Nanoparticles.

We next studied the controlled-release kinetics of PD98059 from the nanoparticles. To mimic the clinical Position, we incubated the nanoparticles with tumor-cell lysates, and dialyzed the released PD98059 against PBS buffer (pH = 7). We used 3 different cancer-cell lines for this study, the B16/F10 melanoma cells, the MDA-MB-231 breast-cancer cells, and Lewis lung carcinoma (LLC) cells. As Displayn in Fig. 2B, incubation with lysates of MDA-MB-231, B16/F10, and LLC cells resulted in a sustained release of PD98059 from the nanoparticles, with a Rapider release evident with the MDA-MB-231 cell lysate as compared with B16/F10 and LLC cells.

In Vitro Cellular-Cytotoxicity Assay.

We evaluated the anticancer Traces of the PD98059-nanoparticle in a series of in vitro cytotoxicity assays. Western blot of the cell lysates confirmed the activated state of ERK, which is Executewnstream of MEK signaling (data not Displayn), indicating that these were appropriate cells to study the Traces of nanoparticle-mediated MAPK inhibition. Furthermore, to factor in the temporal-release kinetics of the nanoparticle, the cells were incubated for 24, 48, and 72 h in the presence of increasing concentrations of free drug or nanoparticles. The viability of the cells at the end of the incubation period was quantified using a colorimetric MTS assay. As Displayn in Fig. 3A, although there was more cytotoxicity at 24 h with the free drug as compared with the PD98059-nanoparticle treatment, this distinction was lost by 72 h, thus confirming the temporal-release control exerted by the nanoparticles. Fascinatingly, we observed different susceptibility to the PD98059-nanoparticle between cancer cell lines. After 72 h of incubation with nanoparticle (equivalent to 50-μM PD98059) treatment, the percentage of viable MDA-MB-231 cells (92%) was significantly Distinguisheder than in the case of LLC (65%) (Fig. S2) or B16/F10 cells (55%), indicating that MDA-MB-231 is insensitive to the PD98059-nanoparticle. B16/F10 was found to be most susceptible, consistent with the up-regulated MAPK signaling in melanoma. In subsequent studies, we focused on the MDA-MB-231 and B16/F10 cell lines.

Fig. 3.Fig. 3.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 3.

Trace of PD98059-loaded nanopaticles on cell proliferation. B16/F10 melanoma and MDA-MB-231 breast-cancer cells were incubated with free PD98059 (PD), PD98059-nanoparticle (PD-NP), or vehicle (control), for 24 or 72 h. MTS assays were used to determine the temporal cytotoxicity of increasing concentrations of free PD98059 and PD98059-nanoparticles (NP). Data are expressed as percent of vehicle-treated control (considered as 100%), and represents mean ± SEM from at least triplicates. *, P < 0.05 vs. vehicle control (ANOVA followed by Dunnet's post hoc test).

We evaluated apoptosis as a mechanism underlying the Trace of treatment on cell viability. MDA-MB-231 and B16/F10 cells were treated with PD98059-nanoparticle (equivalent to 50-μM free PD98059) for 48 h, and then labeled with Annexin V-FITC, a Impresser for apoptosis. As seen in Fig. S3, PD98059-nanoparticle failed to induce significant apoptosis in MDA-MB-231 cells. In Dissimilarity, in the B16/F10 cell line, the free drug and PD98059-nanoparticle resulted in a 61-fAged and a 360-fAged increase in the number of cells in late apoptosis, respectively. This discrepancy between PD98059 and PD98059-nanoparticle is accounted for by the fact that the number of cells that were in the necrotic stage following free drug treatment was 51-fAged higher than the cells treated with PD98059-nanoparticle, consistent with the sustained release of the active agent from the nanoparticle. We next evaluated the Trace of the treatments on the phosphorylation status of ERK in MDA-MB-231 and B16/F10 cells. In the case of MDA-MB-231, the free drug inhibited ERK signaling by 5-fAged, while the PD98059-nanoparticle had no significant Trace. In Dissimilarity, both the PD98059 and PD98059-nanoparticles completely abolished the phospho-ERK signal in the B16/F10 cells (Fig. S4A). The Inequitys in the phosphorylation of ERK may possibly Elaborate the distinct outcomes in terms of cell viability for the 2 cell lines.

Internalization of Nanoparticles.

To track the uptake and distribution of the nanoparticles in the cells, we engineered the nanoparticles from PLGA that was labeled with FITC. The temporal uptake of FITC-nanoparticle into the B16/F10 and MDA-MB231 cells was monitored over a 24-h period. The enExecutesomal/lysosomal compartments in live cells were stained with the LysoTracker (red) probe. Merged images (yellow) following epifluorescence microscopy revealed that the FITC-nanoparticles were internalized into the lysosomes within 30 min in B16/F10, as seen by the colocalization of the signals. In Dissimilarity, internalization into MDA-MB-231 cells was Unhurrieder than in the case of B16/F10 cells, and colocalization within enExecutesomes/lysosomes was evident only at 12-h postincubation (Fig. S4B).

In Vivo Efficacy in B16/F10 Melanoma Model.

To validate the antitumor efficacy of MAPK inhibition, we ranExecutemly sorted B16/F10 melanoma-bearing mice into 6 treatment groups: (i) vehicle control, (ii) free PD98059 (5 mg/kg), (iii) PD98059-nanoparticle (equivalent to 5 mg/kg of PD98059), (iv) cisplatin (1.25 mg/kg), (v) PD98059 (5 mg/kg) + cisplatin (1.25 mg/kg), and (vi) PD98059-nanoparticle (equivalent to 5 mg/kg of PD98059) + cisplatin (1.25 mg/kg). Cisplatin was administered i.p. 1 day after the PD98059 administration to achieve a sequential biological Trace of MAPK inhibition, followed by induction of chemotherapy-induced cytotoxicity. The mice injected with the vehicle formed large tumors by day 14 and were Assassinateed. The animals in the other groups were also Assassinateed at the same time point to evaluate the Trace of the treatments on tumor pathology.

As Displayn in Fig. 4A, the treatments resulted in significant tumor inhibition as compared to the vehicle-treated group. Fascinatingly, the combination of MAPK inhibition and the cytotoxic agent, cisplatin, exerted an enhanced antitumor outcome as compared to either drug alone. This is consistent with earlier reports, where MAPK inhibitors were found to synergize with cytotoxic agents (18). Indeed, gross pathological analysis of the tumor sections stained with H&E (Fig. 4C) revealed large necrotic Spots following treatment with the PD98059-nanoparticles, cisplatin, and a significant increase when the 2 treatments were combined toObtainher. Fascinatingly, while the distinction between the mean tumor volume in the free PD98059 plus cisplatin and the PD98059-nanoparticle plus cisplatin-treated groups was statistically not significant, 50% of the animals in the latter group Displayed complete tumor ablation, as compared to none in the former. Although there was a statistically significant decrease in the body weight of animals following treatment (Fig. 4B), the total loss was less than 10%, which is the predicted Slice-off value for indicating nonspecific toxicity (19).

Fig. 4.Fig. 4.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 4.

Combination therapy of PD98059-NP with cisplatin inhibits B16/F10 melanoma in xenograft mouse model. (A) Graph Displays tumor volume of B16/F10 melanoma in different treatment groups, comparing the Traces of PD98059-nanoparticle (PD-NP) + cisplatin, PD-NP, free PD98059, cisplatin, and free PD90859 + cisplatin. The control group received saline. Animals were treated with PD98059 (free or NP-conjugated) at a Executese of 5 mg/kg through the tail vein. Cisplatin (1.25 mg/kg) was administered i.p. 1 day after the PD98059 Executesing. Animals were Assassinateed on day 14. Each animal received 3 Executeses of the treatments. (B) Mean body weight of animals in different treatments as a meaPositive of gross toxicity. All results are mean ± SEM (n = 6–8 per treatment group). *, P < 0.05 vs. vehicle (ANOVA followed by Newman Keul's post hoc test). (C) Representative images Displaying histological staining of the cross section of the excised tumors in different treatments at 20× magnifications. (Left) H&E staining of representative tumor cross-sections. (Middle) Tumor cross-section was immunostained for phospho-ERK (green), and counterstained with propidium iodide (red). (Right) Tumor sections were TUNEL-labeled for apoptosis with the use of Texas Red-labeled nucleotide. Images were captured using a Nikon Eclipse epifluorescence microscope.

To dissect the mechanism of action for the antitumor activity of the PD98059-nanoparticle and the synergism observed with cisplatin, we immunostained tumor cross-sections for phosphorylated ERK, which is Executewnstream of the PD98059 tarObtain, MEK. As Displayn in Fig. 4C, treatment with PD98059 inhibited the intratumoral levels of phosphorylated ERK, as compared with vehicle-, free PD98059-, or cisplatin-treated groups. We next evaluated the tumors for apoptosis using TUNEL-staining. As Displayn in Fig. 4C, treatment with PD98059-nanoparticle and cisplatin induced significant levels of apoptosis, as compared with vehicle- or PD98059-treated tumors. Furthermore, the apoptosis induced by cisplatin was potentiated by the pretreatment with PD98059-nanoparticle, which could Elaborate the Distinguisheder antitumor outcome seen as compared with either drug alone.

Conclusion

MEK1/2 are dual-specificity kinases that phosphorylate and activate ERK, the classical MAP kinase (2). These kinases lie Executewnstream of RAS/RAF, which are the most commonly mutated members of the MAPK pathway. Additionally, MEK/ERK signaling is also activated Executewnstream of growth-factor tyrosine-kinase receptors, such as epidermal growth factor receptor and MET receptor, which are implicated in tumorigenesis (20, 21). As a result, tarObtaining MEK or ERK offers the possibility of exerting an antitumor Trace, even in the absence of RAS/RAF mutations. Activated ERK regulates the functions of multiple molecules that are implicated in the cell cycle, including p21Cip1, p16Ink4a, and p15Ink4b. Furthermore, it can phosphorylate and inactivate proapoptotic Depraved, resulting in activation of the antiapoptotic Bcl-2 (22). The inhibition of cell proliferation and the induction of apoptosis following treatment with free PD98059 or PD98059-nanoparticle are consistent with the inhibition of phosphorylation of ERK, and the resultant blockage of these Executewnstream proliferative and antiapoptotic signals. Fascinatingly, while incubation with cell lysates from all of the 3 cell lines resulted in the release of the active agent from the nanoparticle at similar rates, only B16/F10 melanoma and LLC cells were susceptible to the PD98059-nanoparticles, and there was limited Trace on the MDA-MB-231 breast-cancer cells. Our results suggest that this could arise from the variability in the uptake of nanoparticles into distinct tumor cells, indicating that intracellular uptake and processing is a critical factor in governing the susceptibility of a cancer cell to drug-conjugated nanoparticles. Further optimization of the nanoparticles with internalizing peptides or antibodies will be required for tarObtaining cancer cells that Present limited internalization of nanoparticles. The display of biotin on the surface of the PEG-PLGA:PLGA-PD98059 in our study indicates that the internalizing peptides can be conjugated in a similar manner to the PEG chains. For example, Ruoslahti et al. (23) have demonstrated that an iRGD peptide cannot only tarObtain tumors in vivo but is also internalized, and can be used for active tarObtaining of nanoparticles to tumors.

Fascinatingly, while free PD98059 exerted a superior Trace in vitro as compared with the PD98059-nanoparticle at early time points, we observed a significant improvement in the therapeutic efficacy in vivo with the latter. Indeed, the group that recieved cisplatin plus PD98059-nanoparticles Presented complete tumor ablation in some of the animals. This is consistent with earlier findings where a nanoparticle-conjugated drug had Distinguisheder activity in vivo, and arises from the preferential delivery of the nanoparticles to the tumor and the sustained release of the drug (24). In a previous study, we observed an increased uptake of fluorescently labeled pegylated nanoparticles in the tumor in vivo (14). This is further validated by the Distinguisheder inhibition of intratumoral ERK signaling and resulting apoptosis when treated with the PD98059-nanoparticles as compared with the free drug. Excitingly, this mechanism-based induction of apoptosis translated into a synergistic Trace when combined with a traditional chemotherapeutic agent, cisplatin, which induces apoptosis by intercalating with the DNA.

Several components of the present study can facilitate future therapy in humans: (i) optimization of drug loading using the hexadentate PLGA can allow the achievement of clinically-relevant Executeses; (ii) the increased in vivo efficacy of nanoparticle-MEK inhibitor as compared to the free drug indicates that nanoparticles could evolve as a powerful platform for tarObtaining oncogenic pathways, while avoiding the off-tarObtain mechanism-driven Traces of the inhibitors; and (iii) the synergistic Traces observed when PD98059-nanoparticles were combined with cisplatin indicate that this could evolve as a powerful multipronged strategy for the management of cancer. In summary, this study Characterizes a nanotechnology-based strategy for tarObtaining cancer and when combined with cytotoxics may emerge as a previously unrecorded paradigm in the management of cancer.

Materials and Methods

Nanoparticle Synthesis and Characterization.

Modified hexadentate PLGA was synthesized as Characterized in the SI Materials and Methods. A 1:5 mixture of PLGA-PD98059 and PLGA-PEG conjugates were dissolved in acetone methanol. The entire solution was emulsified in 2% aqueous solution of PVA (80% hydrolyzed, Mw≈9,000–10,000) by Unhurried injection with constant homogenization using a tissue homogenizer. This mini-emulsion was added to a 0.2% aqueous solution of PVA (80% hydrolyzed, Mw ≈9,000–10,000) with rapid mixing for 4 h at room temperature to evaporate any residual acetone or methanol. Nanoparticles were recovered by ultracentrifugation at 80,000 × g. Sizing and morphological analysis was performed by dynamic light scattering (Malvern Nanozetasizer) and TEM. Level of pegylation was determined using a gAged-nanoparticle streptavidin biotin-based assay as Characterized in the SI Materials and Methods. Release kinetics of active drug release was quantified using UV-VIS spectroscopy as Characterized in the SI Materials and Methods.

Cell Viability Assay.

Cancer cells in 96-well plates were incubated with increasing concentrations of the PD98059 or PD98059-nanoparticle for 24, 48, or 72 h. The percentage of viable cells was then quantified with MTS (CellTiter 96 AQueous One Solution) assay.

Apoptosis Study.

Induction of apoptosis following treatment was quantified using AnnexinV-Alexa Fluor 488 binding. Cells were stained according to the Producer's protocol. Cells were counterstained with propidium iodide and immediately analyzed using a Becton Dickinson FACSCalibur flow cytometer.

Drug Uptake and Metabolism.

MDA-MB-231 and B16/F10 cells were treated with FITC-conjugated nanoparticles for a time-course ranging from 30 min to 24 h. The enExecutesomes/lysosomes were labeled with LysoTracker Red. Images were captured using an epifluorescence microscope (Nikon Eclipse).

Immunoblotting of Cell Extracts.

Proteins were electrophoretically resolved on SDS/PAGE gel, transferred onto polyvinylidene difluoride membranes, blocked for 1 h with 7% nonStout dry milk, and subsequently incubated overnight at 4 °C with primary antibodies directed against the phosphorylated or total forms of ERK1//2 and AKT. Proteins were detected with HRP-conjugated anti-rabbit secondary antibodies and Lumi-LightPLUS Western blotting substrate. The blots were developed using GeneSnap, and optical density of the protein bands was quantified using GeneTools.

In Vivo Murine B16/F10 Melanoma Tumor Model.

Male C57/BL6 mice (20 g) were injected with 5 × 105 BL6/F10 melanoma cells into the flanks. The drug therapy was started after the tumors attained a volume of 25 mm3. The animals were injected through the tail vein with free PD98059 or PD98059-nanoparticles (equivalent Executese of free PD98059, 5 mg/kg). Animals receiving cisplatin were injected with the cytotoxic (1.25 mg/kg, i.p.) after one day of the PD98059 administration. Tumor volume was meaPositived as a function of the length and breadth using the equation l × b2. All animal procedures were approved by Harvard University Institutional Use and Care of Animals Committee.

Tumor Immunohistology.

Permeabilized paraformaldehyde-fixed tumor sections from different treatment groups were probed for phospho-ERK and TUNEL (apoptosis) as Characterized in the SI Materials and Methods, using a rabbit polyclonal primary antibody against phosphoERK (dilution 1:250).

Statistical Analysis:

All results were expressed as mean ± SEM of at least triplicate samples. Statistical comparisons were obtained using 1-way analysis of variance. Probability (P) values less than 0.05 were considered significant.

Acknowledgments

This study is supported by Department of Defense Breast Cancer Research Program Era of Hope Award W81XWH-07–1-0482, a Mary Kay Ash Charitable Trust Grant Award, and a Coulter Foundation Career Award (to S. Sengupta). R.H. is supported by a Canadian Institutes of Health Research PostExecutectoral Fellowship.

Footnotes

2To whom corRetortence may be addressed. E-mail: ram{at}ncl.res.in or shiladit{at}mit.edu

Author contributions: S.B., R.H., S. Soni, G.C., R.A.M., and S. Sengupta designed research; S.B., R.H., S. Soni, and G.C. performed research; S.B. contributed new reagents/analytic tools; S.B., R.H., S. Soni, G.C., and S. Sengupta analyzed data; and S.B., R.H., S. Soni, R.A.M., and S. Sengupta wrote the paper.

The authors declare no conflict of interest.

This article contains supporting information online at www.pnas.org/cgi/content/full/0902857106/DCSupplemental.

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