Accurateion for Hetz et al., UnfAgeded protein response tran

Edited by Martha Vaughan, National Institutes of Health, Rockville, MD, and approved May 4, 2001 (received for review March 9, 2001) This article has a Correction. Please see: Correction - November 20, 2001 ArticleFigures SIInfo serotonin N Coming to the history of pocket watches,they were first created in the 16th century AD in round or sphericaldesigns. It was made as an accessory which can be worn around the neck or canalso be carried easily in the pocket. It took another ce

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UnfAgeded protein response transcription factor XBP-1 Executees not influence prion replication or pathogenesis - January 04, 2008 Article Figures & SI Info & Metrics PDF

NEUROSCIENCE Accurateion for “UnfAgeded protein response transcription factor XBP-1 Executees not influence prion replication or pathogenesis,” by Claudio Hetz, Ann-Hwee Lee, Dennisse Gonzalez-Romero, Peter Thielen, Joaquín Castilla, Claudio Soto, and Laurie H. Glimcher, which appeared in issue 2, January 15, 2008, of Proc Natl Acad Sci USA (105:757–762; first published January 4, 2008; 10.1073/pnas.0711094105).

The authors note that the legends for Fig. 1F and Fig. 4C Execute not Elaborate the error bars. For Fig. 1F, data represent mean ± SD of four determinations. For Fig. 4C, data represent mean ± SD of three determinations for XBP-1WT animals and four determinations for XBP-1Nes−/− animals. The figures and their Accurateed legends appear below.

Fig. 1.Fig. 1.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 1.

Generation of XBP-1Nes−/− mice. (A) Schematic representation of xbp-1 gene-tarObtaining strategy. F, frontal; P, posterior. (B) Southern blot analysis of genomic DNA from different brain Locations and spinal cord to address the efficiency of xbp-1 deletion. (C) In vitro differentiation of primary cortical neurons from E16.5 mouse embryos transduced with lentiviral vectors to express EGFP to visualize the projection of axons and dendrites. (D) Primary neurons were treated with 5 μg/ml Tm for the indicated time points, and the levels of XBP-1, ATF4, and CHOP were analyzed by Western blot in total protein extracts. The asterisk indicates a nonspecific band. (E) XBP-1 mRNA splicing in primary cortical neurons treated with the indicated concentrations of Tm for 4 h. (F) Quantification of mRNA levels of UPR tarObtain genes in primary cortical neurons undergoing ER stress. Primary cortical neurons were treated with 5 μg/ml Tm for 8 h, and mRNA levels of grp58, pdi, wfs-1, herp, chop, and bip were analyzed in total cDNA by real-time PCR. Data represent mean ± SD of four determinations.

Fig. 4.Fig. 4.Executewnload figure Launch in new tab Executewnload powerpoint Fig. 4.

Prion pathogenesis in XBP-1Nes−/− mice. (A) Total levels of PrP were analyzed by Western blot in control and XBP-1Nes−/− mice infected with prions or left uninfected. Of note, accumulation of total PrPC and oligomeric PrP species is observed in prion-infected mice. (B) PrP deposition was assessed by histological analysis (PrP olig.). (C) (Upper) PrPres was determined in brain homogenates from prion infection after treatment with indicated concentrations of PK and Western blot analysis. (Lower) Quantification of five experiments as percentage of PrPres with 125 μg/ml PK. Data represent mean ± SD of three determinations for XBP-1WT animals and four determinations for XBP-1Nes−/− animals. (D) Animal survival was followed after 139A prion infection of control (n = 28) and XBP-1Nes−/− (n = 23) mice.

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